Regulatory

Part:BBa_K1848006:Design

Designed by: Samuel Magaziner   Group: iGEM15_Columbia_NYC   (2015-09-18)


LuxR/AHL driven Lysis Cassette with Protein level Timer Delay


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2246
    Illegal NheI site found at 2269
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1851
    Illegal AgeI site found at 1921
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2502


Design Notes

We had to balance potential basal levels of lysis expression. Luckily, this proved not to be an issue.


Source

The quorum sensing components come from Vibrio fischeri. The cI components hail from the T4 enterobacteria phage.

References